Format du dépôt |
Notice |
Type de dépôt |
Article dans une revue |
Résumé |
en
Interactions between microtubules and viruses play important roles in viral infection. The best-characterized examples involve transport of animal viruses by microtubules to the nucleus or other intracellular destinations. In plant viruses, most work to date has focused on interaction between viral movement proteins and the cytoskeleton, which is thought to be involved in viral cell-to-cell spread. We show here, in Cauliflower mosaic virus (CaMV)-infected plant cells, that viral electron-lucent inclusion bodies (ELIBs), whose only known function is vector transmission, require intact microtubules for their efficient formation. The kinetics of the formation of CaMV-related inclusion bodies in transfected protoplasts showed that ELIBs represent newly emerging structures, appearing at late stages of the intracellular viral life cycle. Viral proteins P2 and P3 are first produced in multiple electron-dense inclusion bodies, and are later specifically exported to transiently co-localize with microtubules, before concentrating in a single, massive ELIB in each infected cell. Treatments with cytoskeleton-affecting drugs suggested that P2 and P3 might be actively transported on microtubules, by as yet unknown motors. In addition to providing information on the intracellular life cycle of CaMV, our results show that specific interactions between host cell and virus may be dedicated to a later role in vector transmission. More generally, they indicate a new unexpected function for plant cell microtubules in the virus life cycle, demonstrating that microtubules act not only on immediate intracellular or intra-host phenomena, but also on processes ultimately controlling inter-host transmission.
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Titre |
en
A role for plant microtubules in the formation of transmission-specific inclusion bodies of Cauliflower mosaic virus
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Auteur(s)
|
Alexandre Martiniere
1
, Daniel Gargani
2
, Marilyne Uzest
2
, Stéphane Blanc
2, 3
, Nicole Lautredou
4
, Martin Drucker
3
1
BPMP -
Biochimie et Physiologie Moléculaire des Plantes
( 538 )
- Bât. 7 - 2 place Pierre Viala - 34060 Montpellier cedex 2
- France
-
Institut National de la Recherche Agronomique UMR 0386 ( 92114 )
;
-
Centre international d'études supérieures en sciences agronomiques UMR 5004 ( 92699 )
;
-
Université de Montpellier UMR 5004 ( 410122 )
;
-
Centre National de la Recherche Scientifique UMR5004 / URA2133 ( 441569 )
;
-
Institut national d’études supérieures agronomiques de Montpellier ( 474617 )
2
UMR BGPI -
Biologie et Génétique des Interactions Plante-Parasite
( 73810 )
- CIRAD
UMR-BGPI TA A-54/K - Campus International de Baillarguet - 34398 Montpellier Cedex 5
- France
-
Centre de Coopération Internationale en Recherche Agronomique pour le Développement UMR54 ( 11574 )
;
-
Institut National de la Recherche Agronomique UMR0385 ( 92114 )
;
-
Centre international d'études supérieures en sciences agronomiques ( 92699 )
;
-
Institut national d’études supérieures agronomiques de Montpellier ( 474617 )
3
LPC -
Laboratoire de Pathologie Comparée
( 1668 )
- Station de recherche Pathologie 30380 ST CHRISTOL LES ALES
- France
-
Institut National de la Recherche Agronomique UMR0027 ( 92114 )
;
-
Université Montpellier 2 - Sciences et Techniques ( 92690 )
;
-
Centre National de la Recherche Scientifique FRE2689 ( 441569 )
4
IGH -
Institut de génétique humaine
( 425 )
- institut de Génétique humaine 141 Rue de la Cardonille 34396 MONTPELLIER CEDEX 5
- France
-
Université de Montpellier ( 410122 )
;
-
Centre National de la Recherche Scientifique UMR9002 / UPR1142 ( 441569 )
|
Localisation géographique du document |
0378 Documentation de centre, Centre de recherche de Montpellier, 34060 Montpellier, FRA (Publis2009/BGPI)
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Commentaire |
Public visé |
Scientifique
|
Version du document |
version éditeur
|
Langue du document |
Anglais
|
Nom de la revue |
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Audience |
Internationale
|
Date de publication |
2009-04
|
Volume |
58
|
Numéro |
1
|
Page/Identifiant |
135-146
|
Voir aussi |
-
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=INRA&SrcApp=INRA&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000264612800011
-
https://archives-publications.inrae.fr/45080.pdf
|
Domaine(s) |
-
Sciences de l'Homme et Société/Etudes de l'environnement
-
Sciences de l'Homme et Société/Economies et finances
-
Sciences du Vivant [q-bio]/Ingénierie biomédicale/Imagerie
|
Indexation contrôlée |
-
légume
-
microtubule
-
virus
-
virus phytopathogène
-
microscopie
|
Mots-clés |
en
CAULIFLOWER MOSAIC VIRUS, FLUORESCENCE IMAGING
fr
PLANT VIRUS, MICROTUBULES, CELL-MICROBE INTERACTION, VECTOR TRANSMISSION, BIOLOGIE CELLULAIRE, VIRUS DE LA MOSAIQUE DU CHOU-FLEUR, TRANSMISSION DU VIRUS
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DOI |
10.1111/j.1365-313X.2008.03768.x |
ProdINRA |
45080 |
UT key WOS |
000264612800011 |