Duffy blood group genotyping in French Basques using polymerase chain reaction with allele-specific primers (PCR-ASP)
Frédéric Bauduer
(1, 2)
,
Mhammed Touinssi
,
Anna Degioanni
(1)
,
Stéphane Leroux
,
Olivier Dutour
(1)
,
Louis Ducout
(2)
,
Philippe de Micco
(3)
,
Jacques Chiaroni
(3)
Mhammed Touinssi
- Fonction : Auteur
Anna Degioanni
- Fonction : Auteur
- PersonId : 11841
- IdHAL : anna-degioanni
- ORCID : 0000-0002-9355-4744
- IdRef : 07723507X
Stéphane Leroux
- Fonction : Auteur
Olivier Dutour
- Fonction : Auteur
- PersonId : 792178
- ORCID : 0000-0001-8134-788X
- IdRef : 029722756
Jacques Chiaroni
- Fonction : Auteur
- PersonId : 748906
- IdHAL : jacques-chiaroni
- ORCID : 0000-0002-3133-8990
Résumé
Blood group antigens such as Duffy represent interesting models for population
genetics studies. The distribution of the Duffy blood group was determined using PCR in a sample of Basque (n ¼ 126) and non-Basque (n ¼ 110) patients fromthe general hospital of the French Basque Country. The frequency of FY*A allele was significantly lower among autochthonous French Basques (P <0.001). This result, obtained for the first time by PCR analysis in this population, was within the range of previous historical studies in various Basque subpopulations using traditional hemagglutination methods. When compared with European data, our Basque sample demonstrated the lowest FY*A/FY*B ratio, this fact confirming hemotypology data published before the molecular biology era. Our results with FY*X were quite similar to those reported in European populations.
genetics studies. The distribution of the Duffy blood group was determined using PCR in a sample of Basque (n ¼ 126) and non-Basque (n ¼ 110) patients fromthe general hospital of the French Basque Country. The frequency of FY*A allele was significantly lower among autochthonous French Basques (P <0.001). This result, obtained for the first time by PCR analysis in this population, was within the range of previous historical studies in various Basque subpopulations using traditional hemagglutination methods. When compared with European data, our Basque sample demonstrated the lowest FY*A/FY*B ratio, this fact confirming hemotypology data published before the molecular biology era. Our results with FY*X were quite similar to those reported in European populations.
Domaines
Anthropologie biologiqueFormat du dépôt | Notice |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
Duffy blood group genotyping in French Basques using polymerase chain reaction with allele-specific primers (PCR-ASP)
|
Résumé |
en
Blood group antigens such as Duffy represent interesting models for population<br />genetics studies. The distribution of the Duffy blood group was determined using PCR in a sample of Basque (n ¼ 126) and non-Basque (n ¼ 110) patients fromthe general hospital of the French Basque Country. The frequency of FY*A allele was significantly lower among autochthonous French Basques (P <0.001). This result, obtained for the first time by PCR analysis in this population, was within the range of previous historical studies in various Basque subpopulations using traditional hemagglutination methods. When compared with European data, our Basque sample demonstrated the lowest FY*A/FY*B ratio, this fact confirming hemotypology data published before the molecular biology era. Our results with FY*X were quite similar to those reported in European populations.
|
Auteur(s) |
Frédéric Bauduer
1, 2
, Mhammed Touinssi
, Anna Degioanni
1
, Stéphane Leroux
, Olivier Dutour
1
, Louis Ducout
2
, Philippe de Micco
3
, Jacques Chiaroni
3
1
UAABC -
Anthropologie Bio-Culturelle
( 942 )
- Faculté de Médecine - Secteur Nord Batiment A - CS80011 Bd Pierre Dramard 13344 MARSEILLE Cedex 15 FRANCE
- France
2
Department of Hematology
( 10324 )
- CH Côte Basque 64100 BAYONNE
- France
3
EFS - Alpes-Méditerranée -
Etablissement Français du Sang - Alpes-Méditerranée
( 121596 )
- 149 bd Baille 13385, Marseille
- France
|
Comité de lecture |
Oui
|
Vulgarisation |
Non
|
Langue du document |
Anglais
|
Nom de la revue |
|
Titre de l'ouvrage |
American Journal of Human Biology
|
Audience |
Internationale
|
Date de publication |
2004
|
Volume |
16
|
Numéro |
1
|
Page/Identifiant |
78-81
|
Titre du congrès |
American Journal of Human Biology
|
Pays |
États-Unis
|
Commentaire |
http://www3.interscience.wiley.com/cgi-bin/fulltext/106571744/PDFSTART
|
Domaine(s) |
|
Mots-clés |
en
Duffy Blood Group, PCR-ASP, French Basques
|
DOI | 10.1002/ajhb.10230 |
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